Isolation and identification of alkaline protease producing fungi from soils of different habitats of Sagar and Jabalpur District (M.P)
نویسنده
چکیده
Proteolytic fungi from the soil samples were isolated on Reese agar plate containing casein as the protein substrate. The mycoflora of different localities of Sagar and Jabalpur districts of Madhya Pradesh were investigated at three different locations viz. Garden, Crop field and Poultry farms. These samples were labeled according to the site of collection as GS (Garden Soil), CFS (Crop Field Soil) and PFS (Poultry Farm Soil). Fifty soil samples were collected from the superficial layer of soil, 5-6 cm depth. Alkaline pH was observed across the sampling sites and a total of 38 samples were found positive for the occurrence of fungi. The total fungal counts of the soils were estimated by standard dilution plate technique. The isolated fungi were identified by their cultural and morphological characteristics. One hundred forty one fungal forms were obtained from the positive soil samples. These included 56 fungal forms from Poultry farm, 47 from Garden soil and 38 from Crop field soils. Greater numbers of species were isolated on dilution plate method as comparison to soil plate method. Using conventional media and techniques, all collection sites yielded populations of filamentous fungi belonging to the phylum Ascomycota (5), Deuteromycota (4), Zygomycota (4). Aspergillus and Fusarium were the most predominant genera.
منابع مشابه
Screening of alkaline protease production by fungal isolates from different habitats of Sagar and Jabalpur district (M.P)
Fungal isolates were screened for alkaline protease production on casein containing agar plates and identified by clear zones of casein hydrolysis around colonies. Totally 141 fungal isolates were used in primary screening of alkaline protease and out of these, only 108 test fungi were found to produce alkaline protease activity as indicated by production of zone of casein hydrolysis around the...
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